Composite

Part:BBa_K353004:Design

Designed by: Alejandro Virrueta   Group: iGEM10_Stanford   (2010-10-25)


I0500 + sRNA2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

We designed the sRNA2 tag. It was developed as to not interfere with E. coli's native genome and to be bound by RSID2 (see part BBa_k353003).


Source

This part is assembled from other parts found in the registry (except for the sRNA sequence).

References